Human free fatty acids (FFA) ELISA Kit

The kit assay Human FFA level in the sample,use Purified Human FFA to coat microtiter plate wells, make solid-phase antibody, then add FFA to wells, Combined FFA antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human free fatty acids (FFA) ELISA Kit